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Synthesis and preparation of HEPPSO buffer 68399-78-0
Category : Other Chemicals/Others
CAS NO : 68399-78-0 EC NO : 269-990-1 MF : C9H20N2O5S MW : 268.3305 Specification : White Powder Packing : 500g/bottle Product description : HEPPSO is a biological buffer with good buffering ability and chemical stability, which can maintain the acid-base balance of solutions in various pH ranges. Therefore, it is often used in molecular biology and biochemical experiments. The synthesis and configuration of HEPPSO will be introduced below. The synthesis method of HEPPSO Firstly, the synthesis of HEPPSO requires a series of chemical reactions to complete. React hydroxyethyl piperazine with 2-bromoacetic acid. This reaction needs to be carried out under appropriate solvent and temperature conditions, with the addition of a certain amount of alkaline catalyst. Then, the obtained reactants are reacted again with sulfonyl chloride to obtain HEPPSO products, which are then dried into powder. Preparation method of HEPPSO Dissolve a certain amount of HEPPSO in an appropriate solvent, commonly used solvents include water, glycerol, and sodium citrate solution. The concentration of the solution can be adjusted according to experimental requirements, and it is generally recommended to be within the range of 10-50mM. During the configuration process, it should be noted that the solubility of HEPPSO is relatively low, so sufficient stirring or ultrasonic treatment is needed to increase its solubility. Precautions for using HEPPSO 1. Select the appropriate HEPPSO concentration based on the required pH range for the experiment. Generally speaking, lower concentrations are suitable for acidic environments, while higher concentrations are suitable for alkaline environments; 2. The buffering capacity of HEPPSO decreases with the increase of temperature, so it is necessary to increase the concentration when used under high temperature conditions; 3. The pH value of HEPPSO buffer solution shifts with temperature changes, so corresponding corrections need to be made according to experimental conditions during use. Hubei Xindesheng Materials is a manufacturer of biological buffering agents. It was not until 2005 that it began to engage in the production and research and development of buffering agents. With rich product experience and a wide range of research and development projects. At present, dozens of biological buffering agents have been developed and produced, including TRIS, HEPES, MOPS, CAPS, BICINE, HEPPSO, etc. And the market feedback is good. It can meet the different usage needs of various industries. If you need to purchase, please feel free to call us for consultation and ordering at any time! Uses : Biological buffer Synonyms : 4-(2-Hydroxyethyl)piperazine-1-(2-hydroxypropane-3-sulfonic acid);HEPPSO N-(2-Hydroxyethyl)piperazine-N-2-hydroxypropanesulfonic acid;HEPPSO;2-hydroxy-3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid;4-(2-Hydroxyethyl)piperazine-1-(2-hydroxy propanesulfonic acid);N-(Hydroxyethyl)piperazine-N'-2-hydroxypropanesulfonic acid; Molecular Structure : 
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Synthesis and preparation of HEPPSO buffer