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Hepes buffer for protein purification
Category : Other Chemicals/Others
CAS NO : 7365-45-9 EC NO : 230-907-9 MF : C8H19N2O4S MW : 239.3119 Specification : White Powder Packing : 500g/bottle Product description : Hepes buffer is a commonly used biological buffer in protein purification analysis. Its appearance is white crystal powder, and it needs to be configured as a solution to maintain and stabilize the pH value of the experimental liquid. Therefore, Hepes has good water solubility, has good buffering ability in the pH range of 6.8-8.2, can control a constant pH value for a long time, and is non-toxic to cells, does not participate in and does not interfere with biochemical reaction processes, It has no inhibitory effect on enzyme chemical reactions, etc. Protein separation and purification is a method of using downstream biotechnology to separate and purify the desired target protein from a mixture. Protein is the fundamental substance of life, and without it, there can be no life. Every cell and important component in the body is involved in proteins. To separate and purify a certain protein, it is first required to release the protein in a dissolved state from the original tissue or cell, and maintain its original natural state without losing biological activity. General protein purification techniques choose suitable biological buffers to extract proteins, and then centrifuge to remove insoluble substances to obtain a crude extract containing the target substance. At this time, a buffer is needed to maintain the pH value of the crude extract. Based on the above understanding of protein extraction and purification, it is found that hepes are very suitable for this experimental project. Because hepes have no toxic effects on cells and do not interfere with related biochemical reactions, they have little interference with the experiment. Hubei Xindesheng Material Technology Co., Ltd. has been researching biological buffering agents since its establishment in 2005, and has a history of nearly 20 years so far this year. Based on market demand and customer feedback, researchers at Desheng continuously optimize product quality and price, successfully producing dozens of biological buffers such as tris, bicine, hepes, CAPS, etc. The purity of the products can reach over 99%, with high purity and good water solubility. If you need it, you can contact me or click on the official website to search for more relevant information! Uses : Biological buffer Synonyms : 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid;4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);HEPES;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade;HEPES;4-(2-hydroxyethyl)-1-piperazineethanesulfonicacid;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;LABOTEST-BB LT00138109;LABOTEST-BB LT00233156;HEPES;2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid;2-[4-(2-hydroxyethyl)piperazin-4-ium-1-yl]ethanesulfonate;2-[4-(2-hydroxyethyl)piperazinediium-1-yl]ethanesulfonate;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid);4-(2-Hydroxyethy1)-1-piperazineethanesulfonic acid;N-(2-Hydroxyethyl)-piperazine-N'-2-ethanesulfonic acid;N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid; Molecular Structure : 
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