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The use of luminol luminescence system
Category : Laboratory Chemicals
CAS NO : EC NO : MF : MW : Product description : Luminol can be oxidized by many substances to produce chemiluminescence, which is the earliest research and most widely used luminescence reagent. The chemiluminescence analysis of metal ions occupies an important position in the entire research and application of chemiluminescence analysis. Some metal ions can enhance the chemiluminescence of the luminol system; some ions can inhibit the chemiluminescence of the luminol system; and some ions can directly oxidize the luminol to produce chemiluminescence. In our drug analysis, we use the catalysis or inhibition of metal ions and compounds for direct or indirect detection. Let's take a look at how luminol "extraordinarily" in drug analysis. Application of Luminol Luminescence System in Pharmaceutical Analysis 1. Direct or indirect detection using the catalysis or inhibition of metal ions and metal ion compounds, such as the determination of sodium nitroprusside (SNP) and catechol compounds. Certain reducing substances such as citric acid, citrate esters, Vc, ascorbic acid, etc. can also be detected by the metal ion-luminol luminescence system. 2. The enhancement or inhibition of the chemiluminescence reaction of luminol by biomolecules and organic substances. DNA, albumin, glucose, and uric acid can enhance the chemiluminescence reaction of luminol, thus establishing their own detection methods. In an alkaline medium, the inhibitory effect of ascorbic acid on the Luminol-K3Fe(CN)6 system can be used to determine ascorbic acid. Luminol-K3Fe(CN)6 series can also be used to monitor glucose and lactic acid. Thiol-containing drugs, promazine, etc. have an inhibitory effect on the luminol luminescence system, and a detection method is proposed accordingly. 3. Preparation of expendable chemiluminescence sensor. Luminol and other reagents are fixed on ion exchange resin to develop a chemiluminescence flow injection sensor, which is used for the determination of Vc. Combine enzyme immobilized column with FIA, use immobilized enzyme reactor, detect by luminol, it has been used for glucose, alcohol, oxalate, lactate, choline, uric acid in serum and urine, etc. Detection. Seeing more of the power of luminol, we suddenly discovered that it is acceptable to us wherever it is. This also fully illustrates its value. If you want to know more about luminol's knowledge and uses, you might as well find its manufacturer-Desheng. I believe you will be worthy of asking. Synonyms : no data
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